. 2004 Jun 16;24(24):5603–5610. doi: 10.1523/JNEUROSCI.0589-04.2004

Table 1.

Regulation of histone modifications at the c-fos, BDNF, and CREB promoters in the hippocampus by acute and chronic ECS

	c-fos			BDNF P2			BDNF P3
Promoter/time course	Acute	Chronic	Acute	Chronic	Acute	Chronic	Acute	Chronic
H4 acetylation
30 min	330 ± 41^a		−220 ± 130^d		156 ± 42		−283 ± 30^b
2 hr	295 ± 53^a	298 ± 71^a	278 ± 60^a	268 ± 50^a	114 ± 23	131 ± 16	490 ± 62^a	−125 ± 19
24 h	−132 ± 32	−205 ± 49^b	185 ± 30^a	−359 ± 79^b	−139 ± 51	−138 ± 14	−134 ± 32	−246 ± 37^b
H3 acetylation
30 min	ND		ND		220 ± 98^a,^c		−351 ± 142^d
2 hr	100 ± 16	295 ± 222^d	−188 ± 86^d	195 ± 43^a	−133 ± 15	−123 ± 11	−265 ± 60^b	−216 ± 69^b,^c
24 h	183 ± 67^d	98 ± 29	ND		339 ± 116^a,^c	411 ± 54^a	153 ± 69	236 ± 47^a,^c
H3 phosphoacetylation
30 min	ND		ND		344 ± 276^d		−239 ± 117^d
2 hr	372 ± 110^a	424 ± 82^a	130 ± 47^d	290 ± 39^a	154 ± 53	−148 ± 24^b	113 ± 31	103 ± 18
24 h	ND		ND		ND		ND
mRNA levels				(total BDNF)
30 min	3237 ± 728^a		185 ± 24^a				107 ± 16
2 hr	1514 ± 418^a		482 ± 44^a				149 ± 19
24 h	131 ± 28	−176 ± 10^b	−123 ± 11	214 ± 24^a			−119 ± 2	−178 ± 31^b

This is a comprehensive report of our data, expressed as percentage increase (a) or decrease (b) over control ± SEM (n = 4−6 animals in each treatment group). The values shown (a or b) represent statistically significant changes (p < 0.05 by paired t test). Percentages (c) indicate trends, changes that did not reach a level of significance (usually because of large variability in the fold increase or the fold decrease among the samples) but in which all samples showed a change in the same direction and were significant by χ² test. The values shown (d) represent conditions for which there was large variability and hence no apparent regulation by ECS. Ct values were too high in some samples, indicating that the anti-histone antibody precipitated negligible levels of that gene and was therefore not detectable (ND).

Increase in acetylation/phosphoacetylation, p < 0.05.

Decrease in acetylation/phosphoacetylation, p < 0.05.

Trend toward increase or decrease (−), p < 0.1.

Large variability among samples, no trend for regulation.