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lhx6 silencing in dissociated cultures from mouse E13.5 brain. Dissociated MGE cultures were transfected with lhx6-directed siRNA-producing vector and pEGFP. A-D, Silencing of lhx6 did not affect expression of GABA (A, B, red) or GAD65/67 (C, D, red), because several siRNA-transfected cells (B, D, green) were positive for these markers (arrows). Scale bars, 50 μm. n = 3 litters.
