Figure 1.

A, PKC theta does not exist in PKC theta –/– mice, whereas PKC α is well expressed. Western blots for muscle tissues and cultured myotubes from PKC theta KO and WT mice were probed with a monoclonal antibody to PKC theta. B, Western blots in A were stripped and reprobed with a monoclonal antibody to PKCα. C, Western blots for PKC theta from tissues: WT ventral spinal cord (VH), whole spinal cord (SC) from KO animals, and CB or glia cells from WT and PKC theta KO animals. Twenty micrograms of total protein were loaded on each lane in A and B, 10 μg for spinal cord tissue and 1 μg for the glia cells.