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. 2004 Apr 14;24(15):3762–3769. doi: 10.1523/JNEUROSCI.3930-03.2004

Figure 6.

Figure 6.

The effect of activity on PMA action in different heterologous culture combinations as revealed by blocking activity with TTX. EPPs were measured in myotubes before treatment (open bars) and again, in the same myotubes, after 2 hr of treatment (filled bars) combining 100 nm PMA and 10-7 m TTX. The preparations were washed with normal growth medium after the treatment and before the second EPP measurement. Note that TTX treatment restores the sensitivity to PMA treatment in the cultures prepared from KO neurons and normal muscle (bars on the right). This is not true for the cultures made from KO muscle and normal nerve. The data labeled Mus KO + TTX were from preparations made with muscle cells from KO animals and nerve and CB from WT animals. Ventral horn (VH) KO preparations were made with VH neurons from KO animals and muscle and CB from WT animals.