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. 2004 Apr 14;24(15):3801–3809. doi: 10.1523/JNEUROSCI.5543-03.2004

Figure 6.

Figure 6.

Accumulation of ApoE and phosphorylated tau in lipid rafts. A, One microliter of fraction 4 at 3, 4, 5, 8, 12, 18, 24, and 28 months was analyzed by immunoblot with antibodies to flotillin, APP, ApoE, WKS46 (aphosphorylation-independent antibody against tau 359–370), and PHF-1 (an antibody to tau phosphorylated at pSer396/pSer404) sites. The amount of flotillin and APP did not change with aging. ApoE increased substantially beginning at 12 months. Total tau detected by WKS46 did not change with aging, whereas phosphorylated-tau stained by PHF-1 increased beginning at 18 months. Sucrose gradient centrifuge fractions (1–11) from AD and control brains were analyzed by immunoblot using anti-flotillin antibody (B), anti-human ApoE antibody (C), or PHF-1 (D). Note the accumulation of ApoE in lipid rafts (fraction 4) from AD as compared with control brains and the intensely labeled smear of phosphorylated tau in lipid rafts from AD but not control brain.