Figure1.

Anisomycin completely blocks protein synthesis in Helix neurons without affecting MAPK-Erk and Jnk pathways. A, Metabolic labeling of cultured neurons with [³⁵S]methionine was performed under control conditions (C), after 2 hr of anisomycin treatment (AM), or 1 hr after AM withdrawal [recovery (R)]. Labeled proteins were separated by SDS-PAGE and detected by autoradiography. Molecular mass markers are shown on the left in kilodaltons. B, The total amount of ³⁵S radioactivity incorporated into SDS-PAGE-resolved proteins is reported as percentage of the respective value of the control group;^*p<0.01 versus control group (n=6); Dunnett's multiple comparison test. C, The immunoblotting of extracts of Helix ganglion neurons incubated for 2 hr in the absence (C) or presence (AM) of anisomycin with phosphorylation state-specific antibodies against MAPK-Erk-1/2 and Jnk-1/2 reveals that the low dose of anisomycin used in the experimental protocol does not affect the state of activation of the Erk and Jnk pathways.