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. 2004 May 5;24(18):4478–4488. doi: 10.1523/JNEUROSCI.0755-04.2004

Figure 5.

Figure 5.

OGD causes a secondary rise in intracellular Cl– that is independent of cell swelling. A, Slices were subjected to OGD, loaded with dihydro-MEQ, and imaged for 10 min at various times during reoxygenation. Values are expressed as the percentage decrease in fluorescence during 10 min from baseline [(ΔF/F) × 100]. Data are the means ± SEM of 29, 9, 6, and 21 cells per condition, respectively. *p < 0.001, **p < 0.01 versus control. B, Slices were superfused with a low-Cl–-containing buffer before imaging at 1 hr after OGD. Data are the means ± SEM of 29, 11, 9, and 4 cells percondition, respectively. *p < 0.001 versus control. C, Slices were imaged at 2 hr after OGD for changes in calcein fluorescence under identical conditions as for MEQ. Values are expressed as the percentage decrease in calcein fluorescence during 10 min from baseline [(ΔF/F) × 100]. Data are the means ± SEM of 14 and 16 cells per condition, respectively. p > 0.05 versus control, NS. All statistical procedures included ANOVA and Tukey's multiple comparison test.