Figure 7.

Effect of OGD on NKCC-1 and KCC2 cotransporter expression in the hippocampus. The hippocampus was dissected from hippocampal slices subjected to OGD and reoxygenated (plus glucose) for 1–2 hr. Samples were prepared for immunoblotting as described in Materials and Methods. A, Representative immunoblots for NKCC1 and KCC2 expression at 1 and 2 hr after OGD. B, Quantitated data from all experiments. Data are the means ± SEM of OD × area digitized normalized to 100. *p < 0.05 versus control; †p < 0.001 versus OGD plus 1 hr of reoxygenation (reox); ANOVA and Tukey's multiple comparison test (n = 4–10 slices for NKCC1 and n = 6–18 slices for KCC2).