Figure 8.

Diazepam attenuates OGD-induced downregulation of KCC2 protein expression. A, Immunodetection of KKC2 protein expression in hippocampal slices. Slices were exposed to diazepam (dz) during reoxygenation and collected 2 hr after OGD. B, Quantification of the immunoblots by densitometry, as described in Materials and Methods. Data are the means ± SEM of 8, 14, and 20 slices per condition, expressed as normalized OD × area. *p < 0.001 versus control; †p < 0.01 versus OGD; ANOVA and Tukey's multiple comparison test.