Table 2.

Effect of OGD and diazepam on the ability of muscimol to increase intracellular Cl^– in area CA1 pyramidal neurons

Condition	Muscimol response (% of control ΔF/Fa)
Standard
Controlb	68 ± 8.6
OGD + 2 hr reoxygenation	-7.3 ± 5.9*
Diazepam
Controlb	42 ± 11.8
OGD + 2 hr reoxygenation	56 ± 11.1

Slices were superfused with muscimol (50 μm) for 10 min at 2 hr after OGD. In these experiments, slices were loaded with dihydro-MEQ 1 hr after OGD. In the diazepam condition, slices were superfused with diazepam (5 μm) for 1 hr during reoxygenation and imaged 1 hr later (no diazepam present). The muscimol response was calculated by expressing the (ΔF/F_muscimol overΔF/F_control) - 1 × 100. Data are the means ± SEM of 16-31 cells per condition. *p < 0.05 versus corresponding control; ANOVA and Tukey's multiple comparison test.

^a This control refers to the absence of muscimol.

^b This control refers to the absence of OGD.