Skip to main content
. 2004 May 5;24(18):4401–4411. doi: 10.1523/JNEUROSCI.0348-04.2004

Figure 1.

Figure 1.

Processing and localization of epitope-tagged neurotrophins. A, Dual N-terminal HA-tagged and C-terminal FLAG-tagged BDNF or NGF was transfected into COS-7 cells, and, after 48 hr, lysates were prepared as described in Materials and Methods and analyzed by immunoblotting with anti-HA and anti-FLAG antibodies. B, Single C-terminal HA-tagged BDNF or NGF was transfected into differentiated PC12 cells. After 48 hr, lysates were prepared as described in Materials and Methods and analyzed by immunoblotting with C-terminal antibodies for BDNF or NGF or with anti-HA antibodies. Dual-epitope-tagged BDNF (C) and NGF (D) were transfected in differentiated PC12 cells. After 48 hr, cells were fixed and permeabilized, and subcellular distribution of neurotrophins was visualized by indirect immunofluorescence microscopy using epitope antibodies. Representative epifluorescence images are shown.