Figure 2.
Variant BDNF has no defect in localization and secretion in endothelial and vascular smooth muscle cells. A, Localization of dual-epitope-tagged BDNF species in endothelial cells. After transfection, fixation, and permeabilization, subcellular distribution of wild-type (Val) and variant (Met) BDNF was visualized by indirect immunofluorescence microscopy using anti-HA and anti-FLAG antibodies. B, Localization of dual-epitope-tagged BDNFVal in vascular smooth muscle cells. After transfection, fixation, and permeabilization, subcellular distribution of BDNFVal (Val) and variant (Met) BDNF was visualized by indirect immunofluorescence microscopy using anti-HA and anti-FLAG antibodies.