Figure 6.
Regulated and constitutive secretion of BDNFVal and BDNFMet. Differentiated (A) and undifferentiated (B) PC12 cells were transfected with BDNFVal (Val), BDNFMet (Met), or both BDNF species (Val/Met). After 48 hr, media was collected under depolarization and constitutive secretion conditions, as described in Materials and Methods, and analyzed by ELISA. Results are presented as a mean ± SEM determined from analysis of three independent experiments (*p < 0.01; **p < 0.001; Student's t test).