Figure 1.

Electrophysiological properties of layer V pyramidal neurons of developing medial prefrontal cortex. A, Morphological reconstruction of one of the pyramidal neurons included in the present study. This neuron from a P12 animal was filled with Alexa 488 and reconstructed from a Z-stack using laser scanning confocal microscopy. B, Resting membrane potential of layer V pyramidal neurons across postnatal development. Note the tendency of these neurons to display more hyperpolarized membrane potentials with increasing age. C, Input resistance of layer V pyramidal neurons across postnatal development. The input resistance of these cells, determined using short hyperpolarizing current pulses in current clamp, markedly decreased during the first 2 weeks of postnatal life.