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Cellular secretion of wild-type and mutant NL1 and NL3. Immunoblots of the cell extract (CE) and medium (M) from transiently transfected FLAG-tagged NL3 and NL1 mutants truncated at positions 639 and 691, respectively. Sixty hours after transfection, cells and culture media proteins were separated by SDS-PAGE under reducing conditions, and neuroligin proteins were visualized with anti-neuroligin-1/3 monoclonal antibody. Molecular weight standards are shown on the left.
