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CCK-2R is expressed in olfactory axons but not GnRH-1 cells in nasal explants. Nasal explant at 7 DIV triple stained for GnRH-1 (green), CCK-2R (red), and olfactory axon antigen NCAM (blue) is shown. GnRH-1 neurons in the periphery of the explant are tightly associated with a network of olfactory fibers. Note that CCK-2R colocalized only with NCAM-positive fibers (arrows; pink) but not with GnRH-1 neurons (arrowheads; green). Scale bars: 20 μm; inset, 5 μm.
