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. 2004 May 19;24(20):4796–4806. doi: 10.1523/JNEUROSCI.5654-03.2004

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Copyright © 2004 Society for Neuroscience 0270-6474/04/244796-11.00/0

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Figure 6. — In vitro analysis of lenti-TrkB.T1. A, Schematic representation of lentiviral vector constructs. Lenti-TrkB.T1 and lenti-GFP vectors were constructed downstream of the CMV promoter. The lenti-TrkB.T1 vector construct contained an HA-tagged TrkB.T1 gene. The lenti-GFP construct contained a reporter gene encoding GFP. LTR, Long terminal repeat; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element. Ba, PC12 cells transfected with TrkB.FL after exposure to BDNF. Bb, PC12 cells cotransfected with TrkB.FL plus TrkB.T1 after exposure to BDNF. Bc, Immunocytochemical analysis of HA immunoreactivity in HEK293T cells infected with lenti-TrkB.T1 virus.