Increased axon bundling in the terminal area of lateral hippocampal axons in the lateral septum in EphB receptor-null mice. Fluoro-ruby was injected into the lateral hippocampus to anterogradely label the lateral hippocampal axons. A-E, Bright-field photomicrographs of five different posterior to anterior coronal sections of a wild-type traced brain showing the major cytoarchitectural features of the septal region. These sections are ∼100 μm apart. Boxes in A-E indicate positions of dark-field panels on the right. F-T, Fluorescence photomicrographs of lateral hippocampal axon terminals at different posterior to anterior positions of wild-type (F-J), EphB3-/- single mutant (K-O), and EphB2-/- single mutant (P-T) mice. ac, Anterior commissure; cc, corpus callosum; DLS, dorsal lateral septum; MS, medial septum; VLS, ventral lateral septum. Scale bars: A, 0.2 mm; F, 0.02 mm.