Figure 9.

Proper hippocampal axon defasciculation is dependent on the cytoplasmic domain of EphB2. Fluoro-ruby was injected into the lateral hippocampus of adult mice that were allowed to survive for 1 week. The traced brains were then sectioned with a vibratome, and the sections were examined for the presence of axon bundles and the morphology of axon terminals in the lateral septum. A, Bright-field photomicrograph of a vibratome section from an adult wild-type mouse. B, Bright-field photomicrograph of a vibratome section from an adult EphB2^-/- mouse. C, D, Bright-field photomicrographs of vibratome sections from two different adult EphB2^ki/ki homozygous mice. E, The axon bundle shown in D labeled with Fluoro-ruby anterograde tracing.