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. 2004 Mar 17;24(11):2837–2845. doi: 10.1523/JNEUROSCI.4789-03.2004

Figure 3.

Figure 3.

IL-1β activates ERM adapter proteins, which localize to the microvillar compartment. a shows confocal images of astrocytes treated with IL-1β as in Figure 1, or left untreated (Ctrl), and stained with phalloidin (red) and an antibody specific for phospho-ezrin (Thr567)/radixin (Thr564)/moesin (Thr558) (green). The experiment shown is representative of three experiments using astrocytes from three different brains. Scale bar, 30 μm. b, Confocal z-series of astrocytes treated and stained as in a were subjected to software rendering. Scale bar, 20 μm. c, Western blots of total cell homogenates treated as shown were probed with antibodies specific for VCAM-1, ICAM-1, phospho-ezrin(Thr567)/radixin(Thr564)/moesin(Thr558), and total ezrin/radixin/moesin. The experiment illustrated is representative of three experiments using astrocytes from three different brains. d, High-power CCD images of microvilli on the surface of astrocytes treated with IL-1β as in (a) and double-stained with antibodies specific for ICAM-1 (red) and phospho-ezrin(Thr567)/radixin(Thr564)/moesin(Thr558) (green). Red channel, green channel, merged, and Kohler images are shown. Scale bar, 4 μm.