Figure 2.

Differential expression of GABA or glutamate in donor cells from the proliferative zone of the MGE or cortex. In this experiment the MGE or neocortex of E14.5 donors was plated onto neonatal cortex feeders and cultured for 2 weeks. Each row of three panels shows identical fields: the left row (B, E, H, K) shows GFP expression by the donor cells, the middle row shows expression of glutamate (C, I) or GABA (F, L), and the right row shows the merged images. Few donors from the MGE (B–D) express high levels of glutamate, whereas many more express GABA (E–G). In contrast, most cortical donors (H–J) strongly express glutamate, whereas few express high levels of GABA (K–M). Scale bar, 50 μm.