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Immunocytochemistry of HEK293 cells transfected with expression constructs for wild-type Nav1.2 (WT) and the truncated mutant proteins R102X tagged with Flag at the C terminus (R102X-FlagC) and cotransfected cells (WT+R102X-FlagC). Each cell was double-stained by anti-Nav1.2 rabbit polyclonal antibody (green) and anti-Flag monoclonal antibody (red). Red fluorescence shows cytoskelton-like filament structures (arrowheads) in R102X-FlagC and WT+R102X-FlagC. Scale bars, 8 μm.
