Figure 3.
Postnatal development of intracellular carbonic anhydrase activity in CA1 pyramidal neurons. a, Specimen recording from an acutely isolated P17 neuron (steady-state pHi 7.3) shows that EZA (30 μm) attenuates the pHi shift induced by the withdrawal of CO2 in the continuous presence of BA (1 μm), indicating the presence of intracellular carbonic anhydrase. b, Data from experiments of the type shown in a quantified as the ratio of the initial ΔpHi/Δt in the presence and absence of EZA. Filled circles, Isolated CA1 pyramidal neurons; open circles, mean of 6–12 CA1 pyramidal neurons in a slice (+SEM). The inset illustrates a pair of isolated, BCECF-loaded CA1 pyramidal neurons from a P15 rat. c, Histogram showing that the percentage of pyramidal neurons expressing intracellular carbonic anhydrase increases steeply at P10–P12.