Figure 4.

Developmental expression of carbonic anhydrase isoform VII as seen by in situ hybridization. a, CAII mRNA is not detected in fluorescence in situ hybridization (a), whereas a steep increase in CAVII mRNA is seen throughout the pyramidal layer of Ammon's horn and in the dentate gyrus by P13 (b). Scale bar, 500 μm. c, Expression pattern of CAVII mRNA demonstrated by radioactive in situ hybridization (left, sense control; right, antisense in P22 slices). Scale bar, 400μm. d, Quantification (mean + SEM) based on the radioactive in situ hybridization shows a steep upregulation of CAVII mRNA between P9 and P15.