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. 2004 Jul 14;24(28):6352–6361. doi: 10.1523/JNEUROSCI.0995-04.2004

Figure 2.

Figure 2.

DHPG-induced increases in the phosphorylation of PDK1, Akt, and mTOR are PI3K dependent. Hippocampal slices were preincubated with either normal ACSF, LY294002 (50 μm), or wortmannin (100 nm) for 30 min and then exposed to DHPG (50 μm) for 5 min in the presence of either normal ACSF or the inhibitors. A, Representative Western blots for phosphorylated PDK1 (p-PDK1), total PDK1, phosphorylated Akt (p-Akt), total Akt, phosphorylated mTOR (p-mTOR), and total mTOR. B, Phosphorylated kinase immunoreactivity was normalized to total kinase immunoreactivity for each kinase. Values are means ± SEM. p-PDK1 values: DHPG, 154 ± 20% of control, n = 6; DHPG plus LY294002, 91 ± 6% of control, n = 3; DHPG plus wortmannin, 103 ± 11% of control, n = 3. p-Akt values: DHPG, 146 ± 19% of control, n = 13; DHPG plus LY294002, 98 ± 18% of control, n = 6; DHPG plus wortmannin, 57 ± 8% of control, n = 6. p-mTOR values: DHPG, 149 ± 20% of control, n = 10; DHPG plus LY294002, 88 ± 27% of control, n = 6; DHPG plus wortmannin, 76 ± 13% of control, n = 6. * denotes statistical significance compared with control with a Student's t test (p < 0.05).