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. 2004 Jul 14;24(28):6352–6361. doi: 10.1523/JNEUROSCI.0995-04.2004

Figure 3.

Figure 3.

The effects of mGluR1 and mGluR5 antagonists on DHPG-induced increases in Akt and mTOR phosphorylation. Hippocampal slices were preincubated with either normal ACSF, LY367385 (LY; 100 μm), a selective mGluR1 antagonist, MPEP (10 μm), a selective mGluR5 antagonist, or LY367385 plus MPEP for 30 min and then exposed to DHPG (50 μm) for 5 min in the presence of either normal ACSF or the antagonists. A, Representative Western blots for phosphorylated Akt (p-Akt), total Akt, phosphorylated mTOR (p-mTOR), and total mTOR. B, Phosphorylated kinase immunoreactivity was normalized to total kinase immunoreactivity for each kinase. Values are means ± SEM. p-Akt values: DHPG, 137 ± 10% of control, n = 12; DHPG plus LY367385, 105 ± 5% of control, n = 9; DHPG plus MPEP, 99 ± 6% of control, n = 9; DHPG plus LY367385 plus MPEP, 93 ± 7% of control. p-mTOR values: DHPG, 164 ± 19% of control, n = 13; DHPG plus LY367385, 121 ± 19% of control, n = 12; DHPG plus MPEP, 119 ± 17% of control, n = 12; DHPG plus LY367385 plus MPEP, 85 ± 6% of control. * denotes statistical significance compared with control with a Student's t test (p < 0.05).