Table 2.
Summary of calcium imaging analysis of GnRH-1 neurons at 7 and 21 div in the presence of TTX
|
|
Number of cells with peaks (%) |
Number of peaks/cell |
Mean peak amplitude |
Cells ≥ 10 peaks (%) |
Percentage of cells contributing to each pulse |
|---|---|---|---|---|---|
| 7 div | |||||
| TTX | 29/31 (94) | 4.2 ± 0.5a | 13.0 ± 1.1a | 0/31 (0)a | 40.6a |
| TTX plus 17α-estradiol | 51/54 (94) | 3.7 ± 0.4a | 13.1 ± 0.4a | 3/54 (6)a | 41.3a |
| TTX plus E2 | 58/59 (98) | 11.5 ± 0.8b | 20.5 ± 0.6b | 34/59 (58)b | 65.8b |
| TTX plus E2 plus ICI | 38/39 (97) | 4.6 ± 0.6a | 17.4 ± 0.9 | 4/39 (10)a | 59.9b |
| TTX plus BSA-E2 | 66/66 (100) | 6.3 ± 0.3a | 21.8 ± 0.5b | 7/66 (11)a | 60.7b |
| TTX plus BSA-E2 plus ICI | 20/21 (95) | 3.7 ± 0.3a | 16.7 ± 0.8a | 0/21 (0)a | 41.3a |
| 21 div | |||||
| TTX | 26/29 (90) | 4.9 ± 0.7 | 10.9 ± 0.8 | 2/29 (7) | 39.8 |
| TTX plus E2 |
21/26 (81) |
2.8 ± 0.6 |
9.7 ± 0.2 |
1/26 (4) |
49.4 |
a
Significantly different from TTX plus E2 within each age.
b
Significantly different from TTX.