Table 3.
Summary of calcium imaging analysis of GnRH-1 neurons after inhibition of gene transcription
|
|
Number of cells with peaks (%) |
Number of peaks/cell |
Mean peak amplitude |
Cells ≥ 10 peaks (%) |
Percentage of cells contributing to each pulse |
|---|---|---|---|---|---|
| TTX plus E2 | 58/59 (98) | 11.5 ± 0.8a | 20.5 ± 0.6a | 34/59 (58)a | 65.8a |
| TTX plus DRB | 26/29 (90) | 5.2 ± 0.8 | 16.7 ± 0.7 | 3/29 (10) | 45.2 |
| TTX plus E2 plus DRB (30) | 41/44 (93) | 2.9 ± 0.3 | 14.1 ± 0.7 | 1/44 (2) | 44.2 |
| TTX plus E2 plus DRB (10) | 38/38 (100) | 3.9 ± 0.3 | 15.1 ± 0.6 | 0/38 (0) | 35.4 |
| TTX plus E2 plus DRB (0) |
38/40 (95) |
5.4 ± 0.5 |
18.1 ± 0.9 |
3/40 (8) |
75.4a
|
a
Significantly different from other treatments (p < 0.05 for ANOVA; p < 0.01 for Χ2).