Figure 6.

Effects of htt bodies on uptake of transferrin. A, MCF-7 cells from the same culture dish were treated with FH969-46Q for 24 hr and incubated with rhodamine-labeled transferrin (TF) at 37°C for the last 30 min. Immunofluorescent Flag labeling (green) is absent from some cells (top left) or is present as a diffuse product (middle left) or in htt bodies (bottom left). TF incorporation (red) is similar in cells without Flag-htt expression or diffuse Flag-htt expression but is markedly diminished in the cell with htt bodies (bottom left). B, Percentage of cells without Flag-htt expression or with Flat-htt expression (diffuse or in htt bodies) that had little or no TF uptake. Approximately one-third of cells with htt bodies formed by either wt or mutant htt were significantly impaired in their capacity to take up or transport transferrin compared with nontransfected cells. Five hundred Flag-positive cells were analyzed on each slide. Bar, mean ± SE. ^*p < 0.05 (Student's t test; n = 4 slides).