Figure 6.

Localization of β-gal-positive cells in E11.5:P23 and E15.5:P20 brains. AdexCAG-NL-LacZ was injected into the midbrain ventricles of embryos on E11.5 and E15.5. These E11.5:P23 and E15.5:P20 brains were fixed and sectioned transversely on a cryostat. The sections were stained for β-gal and with neutral red. Many β-gal-positive cells were observed in the thalamic region of E11.5:P23 brains (A). B shows a high-magnification view of A. The cerebral cortex and hippocampus of an E15.5:P20 brain are indicated in C. The pyramidal neurons in the CA1 and CA2 regions of the E15.5:P20 hippocampus were positive for β-gal. HP, Hippocampus; LG, lateral geniculate nucleus; LV, lateral ventricle; VPM, ventroposteromedial nucleus of the thalamus. Scale bar, 200 μm.