Figure 7.

Clonally related cells are labeled by adenovirus-mediated gene induction on a Z/AP mouse. The system of adenovirus-mediated gene induction in the Z/AP mouse is illustrated in A. Z/AP mice ubiquitously express the LacZ gene under the control of the CAG promoter. When Cre recombinase is introduced into the Z/AP mouse by AdexCAG-NL-Cre (Fig. 1A), it removes the LacZ gene from the chromosomal DNA and a second reporter, the hAP gene, is expressed instead (A). AdexCAG-NL-Cre was injected into the midbrain ventricle of the Z/AP embryo at E11.5. E11.5:E18.5 Z/AP brains were sectioned transversely and stained for hAP (shown in purple). Transverse sections are shown in B-D. Arrowheads and arrows in B-D indicate hAP-positive cells in the VZ and thickly labeled-cells in the marginal zone (MZ) and CP, respectively. LV, Lateral ventricle; IZ, intermediate zone; SP, subplate. Scale bar, 200 μm.