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. 2004 Jan 7;24(1):53–62. doi: 10.1523/JNEUROSCI.4503-03.2004

Figure 1.

Figure 1.

WIN induces inhibition of glutamatergic synaptic transmission in rat anterior VTA DA cell. A, A typical whole-cell patch clamp recording showing that bath application of the CB-R agonist WIN 55, 212-2 (WIN; 1 μm) inhibits NMDA EPSCs amplitude (n = 6; p < 0.0001) when cells are held at +40 mV. Representative traces are shown in the inset. The inset shows single NMDA EPSC from a typical experiment, before (black line) and during (dashed line) superfusion of WIN. Calibration: 100 pA, 10 msec. B, WIN reduces NMDA EPSCs amplitude (n = 6; p < 0.0001), when cells are held at +40 mV. All data are normalized to the respective baseline (5 min of baseline). Black bars show time of superfusion of WIN. SEM bars are smaller than symbols in some cases. The inset shows 10-trace averages of NMDA EPSCs before (black line) and during (dashed line) application of WIN. Calibration: 100 pA, 20 msec. C, WIN reduces AMPA EPSC amplitude (n = 7; p < 0.0001) when cells are held at -70 mV. All data are normalized to the respective baseline (5 min of baseline). Black bars show time of superfusion of WIN. SEM bars are smaller than symbols in some cases. Representative traces of AMPA EPSCs before and during application of WIN are shown in the inset. Calibration: 100 pA, 20 msec. D, WIN enhances the paired-pulse ratio of AMPA EPSCs (from EPSC2/EPSC1 = 1.07 ± 0.2 to EPSC2/EPSC1 = 2.06 ± 0.5; n = 7; p < 0.0005). The left-hand graph plots the paired-pulse ratio for each of the experiments in C before (basal) and after (WIN) the application of WIN, whereas the right-hand graph plots the averaged paired-pulse ratio in a bar graph form. Representative traces are shown in the inset. Calibration: 100 pA, 20 msec.