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. 2004 Aug 4;24(31):6958–6967. doi: 10.1523/JNEUROSCI.1331-04.2004

Figure 2.

Figure 2.

Striatal neuron differentiation of LGE-derived neurospheres differentiated in coculture with age-matched primary LGE cells. A-C, Neurospheres originating from LGE of GFP-expressing transgenic embryos, differentiated into ISL1-expressing cells when differentiated in coculture with primary LGE cells, as detected by coexpression of ISL1 and GFP (arrows). Arrowheads show neurosphere-derived cells not expressing ISL1. D-F, Some neurosphere-derived cells also expressed DARPP-32. G-L, Expression of ISL1 and DARPP-32 in LGE-derived neurospheres was confirmed using neurospheres generated from wild-type mice and labeled with a GFP-expressing retrovirus. M, N, Neurosphere-derived ISL1+ cells were not generated at the expense of Er81+ neurons, because differentiation in coculture with primary LGE cells induced the expression of ISL1 but did not change the percentage of neurosphere-derived Er81+ neurons. O, RA and BDNF increase the number of DARPP-32 expressing neurons in the cocultures. Error bars indicate SEM. Scale bars, 50 μm.