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. 2004 Aug 25;24(34):7491–7502. doi: 10.1523/JNEUROSCI.1255-04.2004

Figure 2.

Figure 2.

The C terminus of stargazin exclusive of the last 12 amino acids is necessary for AMPAR synaptic targeting. Constructs encoding stargazin-GFP (star-GFP), stargazin(Δ319-323)-GFP [star(Δ319-323)-GFP], or stargazin(Δ204-311)-GFP [star(Δ204-311)-GFP] were transfected into neurons, which were then fixed (at 11-17 d in vitro) and stained for GFP (green) and GluR1 (red). GluR1 remains clustered at synaptic sites in neurons transfected with stargazin-GFP (A-C, J). In neurons transfected with stargazin(Δ319-323)-GFP, GluR1 shows reduced clustering compared with adjacent untransfected cells (D-F, J). Similar to the reduction of GluR1 clustering seen with the construct lacking the PDZ-binding domain, clustering of GluR1 is also reduced in neurons transfected with stargazin(Δ204-311)-GFP (G-J). Merged images are shown in C, F, and I. The white boxes in C, F, and I are enlarged and presented in J. Scale bar, 10 μm.