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. 2004 Aug 25;24(34):7491–7502. doi: 10.1523/JNEUROSCI.1255-04.2004

Figure 4.

Figure 4.

nPIST localizes to the TGN and colocalizes with stargazin when cotransfected in COS-7 cells. COS-7 cells were transfected with the indicated constructs and immunostained as indicated. A, Stargazin (red) transfected alone shows diffuse membrane and perinuclear staining. B-E, nPIST-GFP (green) transfected alone shows diffuse staining as well as perinuclear accumulations that colocalize with the trans-Golgi network marker, TGN38 (red). F-H, When stargazin (red) is expressed with nPIST-GFP (green), stargazin accumulates with nPIST-GFP in a perinuclear compartment. I-K, An nPIST construct that lacks a complete PDZ and AC domain [nPIST(1-312)-GFP, green] also accumulates stargazin in a perinuclear compartment. In contrast, cotransfection of stargazin (red) with an nPIST construct lacking the predicted stargazin binding site [nPIST(289-463)-GFP, green] (L) or transfection of nPIST-GFP (green) with a stargazin construct lacking the nPIST binding site [stargazin(1-243)] (M) results in no perinuclear colocalization. Scale bar, 10 μm.