Figure 5.

nPIST is enriched in developing and adult rat brain. A, In situ hybridization of sagittal sections of embryonic day 16 rat embryos shows that nPIST is expressed in diverse populations of neurons and is enriched in brain, dorsal root ganglia (DRG), and trigeminal ganglia (TG). Both nPIST and stargazin occur at high levels in the cerebellum (CBL), hippocampus (HC), and cerebral cortex (CTX) in postnatal day 0 (P0) (B), P3 (C), and P30 (D, E) rat brain. F, Western blotting using anti-PIST antibodies shows widespread PIST expression with enrichment in brain tissues, corresponding to the unique presence of the neuronal isoform nPIST in the brain. G, Immunohistochemistry shows high levels of nPIST expression in the hippocampus (HC), cortex (CTX), and cerebellum (CBL), consistent with in situ hybridization data. H, Higher-power magnification demonstrates higher nPIST expression in the Purkinje cell layer (Pu) than the molecular (Mol) or granule cell (Gr) layer of the cerebellum. I, In the hippocampus, nPIST staining is enriched in the pyramidal cell layers CA1, CA2, and CA3.