Figure 7.

pERK is habituated by repeated playback. A, Representative immunoblots of zebra finch AL extracts probed with pERK-specific and cyclophilin antibodies as in previous figures. AL was collected from birds exposed to eight (A8) or 908 × (A908) repetitions of a single song or to 900 repetitions of one song, followed by eight repetitions of a different song (A900B8). B, Group data of corrected pERK signal in AL from birds under conditions A8 (n = 8), A908 (n = 6), and A900B8 (n = 6) shown as fold increase relative to average intensities of corrected pERK in silence controls (n = 8). Gray fill indicates statistically significant difference from silence controls (p < 0.05, one-way ANOVA and Dunnett's t test). Note that extended song repetition (A908) led to suppression of pERK, but brief exposure to a novel song then reactivated pERK phosphorylation (A900B8). Error bars represent SEM.