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. 2004 Jul 7;24(27):6133–6143. doi: 10.1523/JNEUROSCI.0600-04.2004

Figure 3.

Figure 3.

Preferential expression of D1-type receptors by Treg. Expression of dopamine receptors on Treg and Teff was investigated on mRNA and protein levels. a, b, Semiquantitative reverse transcription-PCR (RT-PCR) analysis for D1-R and D5-R expression. mRNA was extracted from freshly isolated Teff and Treg. The housekeeping gene β-actin was used for quantitative analysis. The results of one of five representative experiment are shown. c, Quantitative real-time PCR using primers for D1-R and D5-R to verify the differences in the expression of dopamine receptors on Teff and Treg. The results presented are arbitrary units and are from one of three representative experiments performed. d, e, Semiquantitative RT-PCR for D2-R, D3-R, and D4-R expression. mRNA was extracted from freshly purified Teff and Treg. The housekeeping gene β-actin was used for quantitative analysis. The results of one of five representative experiments are shown. f, Representative micrographs of D1-R-immunoreactive T-cells using fluorescence and confocal microscopy. Also shown are micrographs stained with Hoechst and visualized by fluorescence microscopy. D1-R immunoreactivity was observed in Treg but not in Teff.