Table 2.
Decreased hippocampal cell number in Fgfr1 conditional null mice
|
Embryonic and perinatal mice |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total number of cresyl violet-stained cells (× 103) |
||||||||||||
| Hippocampal VZ |
Hippocampal DG |
|||||||||||
| Genotype |
E16.5 |
P0 |
P7 |
E16.5 |
P0 |
P7 |
||||||
| Fgfr1flox/flox | 156.5 | 377.9 | 452.9 | 152.5 | 598.5 | 637.6 | ||||||
| hGFAP-Cre/+; Fgfr1flox/flox | 189.2 | 178.2 | 304.4 | 131.1 | 381.5 | 224.0 | ||||||
| Percentage decrease |
— |
52.9 |
32.8 |
14.1 |
36.3 |
64.9 |
||||||
| Adult mice |
||||||||
|---|---|---|---|---|---|---|---|---|
| Total number of immunostained cells (× 103) |
||||||||
| Hippocampal CA fields |
Hippocampal DG |
|||||||
| Genotype |
NeuN |
PV |
NeuN |
PV |
||||
| Fgfr1flox/flox | 697 ± 74.8 | 26.0 ± 1.6 | 614.2 ± 57.6 | 5.94 ± 1.16 | ||||
| hGFAP-Cre/+; Fgfr1flox/flox | 339 ± 26.5*** | 13.0 ± 1.0*** | 437.3 ± 14.6* | 2.09 ± 0.57* | ||||
| Percentage decrease |
51.4 |
50.0 |
28.8 |
64.8 |
||||
Values were obtained by unbiased stereological analyses in serial sections stained as indicated. For cresyl violet staining at the three perinatal ages shown, a total of six mice per genotype was statistically analyzed by ANOVA, which showed an overall significant effect of genotype on cell number (p < 0.001). For the NeuN-and parvalbumin (PV)-stained sections of adult mice, three brains per genotype were analyzed. ANOVA showed an overall significant effect of genotype on NeuN cell number (F = 29.0; p < 0.001) and on PV cell number (F = 54; p < 0.0001). Post hoc Sheffe tests showed that control mice significantly differed from mutants in both regions (*p < 0.05; **p < 0.01; ***p < 0.001).