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. 2004 Sep 22;24(38):8346–8353. doi: 10.1523/JNEUROSCI.1809-04.2004

Figure 2.

Figure 2.

Upregulation of purpurin mRNA after optic nerve transection. A, Northern blot hybridization of purpurin mRNA. Top panel, Autoradiograms of purpurin mRNA at various times (days) after optic nerve transection. Middle panel, Autoradiograms of α-tubulin mRNA. Northern blot hybridization was conducted using cDNA probes of purpurin and α-tubulin. Bottom panel, Staining pattern of ethidium bromide (Et Br) of gel. B, Early and transient increase in purpurin mRNA after optic nerve transection. The relationship between purpurin mRNA and the time after optic nerve transection is plotted. The values are presented as the mean ± SD of three independent experiments. C, Retina-specific expression of purpurin mRNA. Total RNAs from the retina (left) and tectum (right) at 0 and 5 d after optic nerve transection were loaded onto agarose/formamide gel.