Figure 7.

Time course of neurite outgrowths in the retinal explant culture under various conditions. A, No addition, control retina (○); addition of anti purpurin IgG (5–10 μg; •); addition of preimmune IgG (5–10 μg; ×). B, No addition, primed retina (underwent optic nerve transection 5–7 d previously; ○); addition of anti purpurin IgG (5–10μg;•); addition of preimmune IgG (5–10 μg; ×). C, Addition of purpurin (1 μg/ml; □) or purpurin (1 μg/ml) with retinol (1 μm; ▪). D, Addition of retinoic acid (1 μm; ▴); purpurin with retinol and disulphiram (10 μm; ▵); retinoic acid (1 μm) with disulphiram (10 μm; ×). E, Neurite outgrowth with the addition of purpurin and retinoids to the control retina during 5 d of culture. Note a drastic enhancement of neurite outgrowth was seen with the addition of purpurin with retinol or retinoic acid. Note a complete blockage of the neurite-promoting effect of purpurin with retinol by addition of disulphiram (*p < 0.01 vs control and **p < 0.01 vs purpurin with retinol).