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. 2004 Sep 22;24(38):8300–8309. doi: 10.1523/JNEUROSCI.2893-04.2004

Figure 4.

Figure 4.

a-j, NGF induces activation of AKT and ERK in cultured DRG neurons. a-f′, Bath application of NGF (100 ng/ml) to DRG cultures results in phosphorylation of AKT at Ser473 (pAKT-S; a, b) and ERK (d, e). The induction of pAKT-S (c) and pERK (f) is abolished by LY294002 (100 μm; LY). a′-f′ are images of corresponding to a-f with high contrast to show all of the cells in each field. Scale bar, 50 μm. The DRG cultures were grown for 24 hr and stimulated with NGF for 10 min. g, NGF does not change AKT phosphorylation at Thr308 (pAKT-T). h, i, Intensity of pAKT-S and pERK immunofluorescence. NGF-evoked pAKT-S (h) and pERK (i) induction is effectively blocked by LY294002 (100 μm). *p < 0.01, compared with control; +p < 0.01, compared with NGF group (n = 4). j, Western blot confirms that NGF-induced increase in pERK levels is blocked by LY294002 (100 μm). Total AKT levels were used as loading controls. Cont, Control.