Figure 4.

Postembedding in situ hybridization electron microscopy in the dentate gyrus after pilocarpine seizures. BDNF mRNA is targeted to dendrites of dentate gyrus granule cells. A, Three hours after pilocarpine administration, gold grains for BDNF mRNA can be visualized in dentate gyrus dendrites associated with a large number of polyribosomes at dendritic hillock (A, B), proximal dendrite (A, C), and dendritic branchings at more distal dendritic domains (A, D). A few grains are also associated with microtubules (A, C, D). Scale bars: A, 2 μm; (in D) B-D, 1 μm.