Figure 2.

Conventional PKCs are activated by MAG and Nogo. a, Representative (n = 4) image that shows activation of PKC by MAG and Nogo in cultured cerebellar neurons. Note that the activation of PKC (phosphorylated) is abolished by pretreatment with PTX. MAG, MAG-Fc (25 μg/ml); Nogo, the Nogo peptide (4 μm). b, Representative image (n = 3) that shows phosphorylation of PKC-α by MAG-Fc in cultured cerebellar neurons. The bottom panel shows total PKC-α. c, Representative image (n = 3) that shows phosphorylation of PKC-α, -β1, or -β2 by MAG-Fc in cultured cerebellar neurons. Each isoform of conventional PKCs is immunoprecipitated (IP) with specific antibody and blotted with anti-phosphorylated pan-PKC antibody. Cont, Control.