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. 2004 Jul 28;24(30):6826–6832. doi: 10.1523/JNEUROSCI.1856-04.2004

Figure 3.

Figure 3.

MAG and Nogo stimulate neurite outgrowth when PKC is inhibited. a, Neurite outgrowth of cerebellar neurons. MAG, MAG-Fc (25 μg/ml); Nogo, the Nogo peptide (4 μm); PTX, PTX (2 ng/ml); U73122, U73122 (20 nm). Data are mean ± SE. b, Dissociated cerebellar neurons were incubated for 24 hr with or without MAG-Fc and the PKC inhibitor peptide. Neurons were then immunostained with monoclonal antibody (TuJ1) for the purpose of recognizing the neuron-specific β-tubulin III protein. MAG, MAG-Fc (25 μg/ml); PKCI, the PKC inhibitor (2 μm). c, Neurite outgrowth of cerebellar neurons. MAG-Fc as well as the Nogo peptide stimulates neurite outgrowth in the presence of the PKC inhibitor. MAG, MAG-Fc (25 μg/ml); Nogo, the Nogo peptide (4 μm); PKCI, the PKC inhibitor (2 μm); HA1077, a Rho-kinase inhibitor (10 mm); PKCI(θ), myristoylated PKCθ pseudosubstrate (2 μm). Data are mean ± SE. Asterisks indicate statistical significance. *p < 0.01 (Student's t test). d, Representative figure showing that activation of PKC (phosphorylated) by MAG-Fc is abolished by pretreatment with the PKC inhibitor (2 μm) Cont, Control.