Figure 4.

PKC regulates myelin-elicited growth cone collapse. a, Growth cone collapse assays. E12 chick DRG explants were treated with MAG-Fc (25 μg/ml) in the presence or absence of the PKC inhibitor (PKCI; 2 μm). Note the prominent spreading of growth cones induced by MAG-Fc in the explant pretreated with the PKC inhibitor. b, Results of growth cone collapse assays. We used 0.1-10 ng/μl CNS myelin for the treatment. MAG, MAG-Fc (25 μg/ml); Nogo, the Nogo peptide (4 μm); PKCI, the PKC inhibitor peptide (2 μm). Data are mean ± SE. Asterisks indicate statistical significance. ^*p < 0.01 (Student's t test).