Figure 5.

PKC is independent of Rho activation. a, Neurite outgrowth of cerebellar neurons. MAG, MAG-Fc (25 μg/ml); Nogo, the Nogo peptide (4 μm); XestC, XestC (1 μm). XestC enhanced the effect of MAG-Fc or the Nogo peptide. Data are mean ± SE. Asterisks indicate statistical significance. ^*p < 0.01 (Student's t test). b, Affinity precipitation of RhoA in cerebellar neurons. RBD, Rho binding domain. MAG-Fc and the Nogo peptide activate RhoA in the presence or absence of the PKC inhibitor. MAG, MAG-Fc (25 μg/ml); Nogo, the Nogo peptide (4 μm); PKCI, the PKC inhibitor peptide (2 μm). These results were reproducible (n = 3). Cont, Control.