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. 2004 Apr 7;24(14):3600–3609. doi: 10.1523/JNEUROSCI.1134-03.2004

Figure 5.

Figure 5.

Calphostin C, a PKC inhibitor interacting with the regulatory domain, blocks the induction but not the maintenance of site-specific ITM. A, Mean ± SEM normalized duration of T-SW over time in preparations treated with the PKC inhibitor calphostin C (5μm) or vehicle (1% DMSO) for 45 min before and during training with a single shock to the test site (n = 6 per group). Calphostin–vehicle was added to the ring ganglia subchamber after completion of the pretests and was washed out immediately after training. Calphostin C completely blocked the induction of site-specific ITM. B, Mean ± SEM normalized duration of T-SW over time in preparations treated with 5 μm calphostin C (Calph; n = 6) or vehicle (Veh; n = 8) beginning 30 min after training with a single shock to the test site. Nontrained preparations treated with calphostin C over the same time period (n = 3) were examined in parallel. Calphostin–vehicle was washed out immediately after the 75 min post-test. Drug–vehicle application is indicated by shading. The maintenance of site-specific ITM was not affected by calphostin C. Note that the calphostin C incubation time in this experiment is identical to that in A. Pre1–Pre3, Pretests 1–3.