Figure 5.

Trafficking of βIV spectrin is not altered in qv^3J mice. Double labeling of WT or qv^3J optic (A-D) and sciatic (E-H) nerves using βIV NT (N-terminal; A, C, E, G) and Pan Nav (A′, C′, E′, G′) or AnkG (B, D, F, H) and Nav1.6 (B′, D′, F′, H′) antibodies. Double immunostaining of WT (I, I′) and qv^3J (J-L) sciatic nerve nodes of Ranvier withβIV NT (I-L) and anti-Caspr antibodies (I′, J′, K′, L′). In qv^3J opticnerve, βIVNT immunoreactivity is undetectable. However, in the qv^3J PNS, βIVNT was detected in reduced amounts at ∼40% of nodes (G, K, L). AnkyrinG immunoreactivity was detectable in qv^3J nodes but reduced in amount and in broader clusters in the CNS (D, arrows). Compared with WT mouse initial segments (M), Nav 1.6 immunoreactivity was reduced at axon initial segments in qv3J mutant mice (N). Scale bars: A-H, 3 μm; I-L, 5 μm; M, N, 10 μm.