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. 2004 Aug 18;24(33):7272–7276. doi: 10.1523/JNEUROSCI.2306-04.2004

Figure 2.

Figure 2.

Effects of treatment with different detergents on the association of FMRP with polyribosomes. A, Cortical extracts were prepared from 8-d-old mice in complete absence of detergent and analyzed as in Figure 1. B, Treatment with deoxycholic acid disrupts the interaction of FMRP with polyribosomes. Cytoplasmic extracts from mouse brain cortex were treated with 1% NP-40 (left panel) or 1% NP-40 and 0.5% deoxycholic acid (right panel). Subsequently, the extracts were fractionated on a 20-50% w/w sucrose linear density gradient. Fractions were collected and analyzed by Western blot using anti-FMRP (IC3) and anti-L7 antibodies.